Lingual adaptations of the Tarentola annularis with new insights into its papillary system adaptations: Ultrastructure, histochemistry, and immunohistochemical observations.

Our research aims to conduct a comprehensive ultrastructural, histochemical, and immunohistochemical examination of Tarentola annularis' tongue, utilizing various techniques such as light, scanning electron microscopy, and morphometric analysis. The complex papillary system consisted of four conical subtypes and one filiform type. The apex carried three conical subtypes (elongated, quadrilateral, and round); the midtongue carried two papillary types (quadrilateral conical and rectangular pointed filiform); and the hindtongue carried two conical subtypes (quadrilateral and elongated serrated). The dorsal papillary surface carried little taste pores on the foretongue and taste buds on the midtongue. The foretongue had a slightly stratum corneum that spread to coat the papillae, while the mid- and hindtongue did not. The glands are absent from the foretongue but are found in the interpapillary spaces of the mid- and hindtongue. Histochemical analysis reveals the presence of collagen fibers in the muscle bundles and the papillary core. The midtongue glands exhibited a strong reaction to AB and PAS, while the hindtongue showed moderate AB positivity and strong positive PAS. The cytokeratin expression in the foretongue papilla was positive, whereas the papillae in other regions were negative. The Tarentola annularis exhibits distinctive lingual structural characteristics due to its varied feeding habits influenced by available food particles.

Age-related morphological and ultrastructural changes in the palate and pharyngeal masticatory apparatus of grass carp (Ctenopharyngodon idella) juveniles.

Grass carp (Ctenopharyngodon idella or C. idella) is a Cyprinid fish frequently utilized for aquaculture, medical, and research purposes. In C. idella, the palate is followed by a well-developed pharyngeal masticatory apparatus. The latter consists of an upper chewing pad superimposing a paired set of teeth. The present study investigated morphological, morphometric, histochemical, and surface ultrastructural changes involving these structures in C. idella juveniles at three different timepoints namely 35 mm total length (TL; 59 days posthatching (dph); fry-fingerling transition), 70 mm TL (90 dph; fingerling), and 210 mm TL ( 365 dph: yearling). The palatal epithelium revealed a constant number of taste buds. However, the height and width of these buds revealed an age-dependent increase. The number of palatal acidic goblet cells increased gradually with age. Enhanced keratinization of pad epithelium, and increased teeth dimensions were age-associated characteristics. Ultrastructurally, the palatal surface of C. idella was slightly papillated at 35 mm TL after which it formed brick-like structural units that tended to cluster into longitudinally paralleled rows toward the palate-pad junction. Goblet cell openings appeared oval at 35 mm TL and became club-shaped by 210 mm TL at which the epithelium appeared compact and heavily coated in mucus. Indentations of pad surface and signs of dental wear and tear were evident ultrastructurally at 70 mm TL and onwards. The current study reports for the first time age-related developmental features of the palate and pharyngeal masticatory apparatus of grass carp. Results of the present work will help to understand aging-associated factors involving the studied fish and other related aquatic species.

Tongue of the Egyptian Endemic Bridled Skink (Heremites vittatus; Olivier, 1804): Gross, Electron Microscopy, Histochemistry, and Immunohistochemical Analysis.

The present study used light and scanning electron microscopy to describe the integrative morphological description of the tongue and laryngeal mound of Heremites vittatus, an endemic lizard of Saharan Africa. Additionally, ultrastructure, histology, histochemistry, and immunohistochemical approaches were used to characterize the lingual apparatus adaptations. In the present study, Heremites vittatus consisted of a complex lingual papillary system in which the ventral apical surface of the foretongue comprised conical papillae. The dorsal surface consisted of different filiform papillary (papillae filiformes) types: the anterior section had two types (bifid and pointed), and the posterior section had four types (triangular, trifid, quadrifid, and pentafid) papillae. The dorsal midtongue surface exhibits scale-like, serrated filiform papillae with anterior gland openings. The hindtongue consisted of two overlapping filiform papillae: scale-like, board-serrated papillae on the median portion and finger-like papillae on the wings. The dorsal surface of the laryngeal mound had 18 longitudinal folds with glandular openings. Histologically, the foretongue was covered by a slightly keratinized layer that was absent in the mid- and hindtongue. The lingual glands were absent from the foretongue but present in the interpapillary space in the mid- and hindtongues. We observed a few rounded taste buds in the conical papilla epithelium. Histochemical analysis revealed strong glandular Alcian Blue (AB)-positive and Periodic Acid-Schiff (PAS)-positive reactions. Immunohistochemistry showed strong cytokeratin immunopositivity in all parts of the tongue. In conclusion, the obtained data about the lingual characterizations have been consistent with the active foraging behavior of the species and its environmental conditions.

Histological and ultrastructural characterization of the dorso-ventral skin of the juvenile and the adult starry puffer fish (Arothron stellatus, Anonymous 1798).

BACKGROUND: The starry puffer fish (Arothron stellatus, Anonymous, 1798) is a poisonous tetradontidae fish inhabiting the Red sea. The skin constitutes an important defense against any external effects. The study aims to characterize the dorso-ventral skin of the juvenile and the adult starry puffer fish using light and scanning electron microscopies. Twenty specimens of juvenile and adult fresh fishes were used. RESULTS: The scanning electron microarchitecture of the skin of the juvenile and adult fish showed delicate irregular-shaped protrusions, and well-defined bricks-like elevations on the dorsal side and interrupted folds as well as irregular-shaped protrusions on the ventral side. In adult fish, the patterned microridges of the superficial and deep epithelial cells (keratinocytes) were larger and well-defined in the dorsal skin than in the ventral side, the contrary was seen in the juvenile fish. The microridges were arranged in a fingerprint or honeycomb patterns. The openings of the mucous cells were more numerous in the dorsal skin in both age stages but more noticeable in adult. Furthermore, the sensory cells were more dominant in the juveniles than the adults. The odontic spines were only seen in adult. Histologically, few taste buds were observed in the epidermis of the dorsal skin surface of the adult fish. Both mucous and club cells were embedded in the epidermis of the juvenile and adult fish with different shapes and sizes. Melanophores were observed at the dorsal skin of both juvenile and adult fishes while fewer numbers were noticed at the ventral surfaces. Several dermal bony plates with different shapes and sizes were demonstrated in the skin of both adult and juvenile fishes. CONCLUSION: The structural variations of skin of the juvenile and adult fishes may reflect the various environmental difficulties that they confront.

Preen gland of the laughing dove (Streptopelia senegalensis aegyptiaca): Light and electron microscopic analysis.

This work reviews the microscopic anatomy of the preen gland in laughing dove (Streptopelia senegalensis aegyptiaca) using light, scanning, and transmission electron microscopes. The gland possessed two large pea-shaped lobes. The glandular lobules of each lobe were huddled in elliptical, triangle, round shapes, connected with each other by strands of connective tissue septae. The lobule was composed of glandular follicles, each follicle folded and enclosed by a sheath of connective tissue connected with the neighboring ones by interfollicular septae. The gland's parenchyma was coated with a dense connective tissue capsule composed of collagen, elastic, and reticular fibers. The secretory tubules were divided into peripheral tubules and central tubules. The central ones were located close to the major cavity and lined with thin epithelium, whereas the peripheral tubules were adjacent to the capsule and characterized by taller epithelium. The central secretory tubules were lined with four cell layers: flattened basal, large-sized polyhedral intermediate, and secretory cell layers, as well as a degenerative cells layer that formed of small cells with pale cytoplasm and pyknotic nuclei. Variable sizes and shapes of Herbst corpuscles were detected alongside the papillary duct and near the glandular lobe. Transmission electron microscopy view revealed that the cytoplasm of the intermediate cells contained a dense population of mitochondria, while the secretory and degenerative cells contained fewer mitochondria. In conclusion, these structures will be beneficial for understanding the habitat differences of laughing doves. RESEARCH HIGHLIGHTS: Grossly, the preen gland (PG) of the laughing dove formed of two large pea-shaped lobes. The glandular lobule was composed of glandular follicles, each follicle was folded and enclosed by a sheath of connective tissue connected with the neighboring ones by interfollicular septae. The central secretory tubules were lined with four cell layers: basal, intermediate, secretory, and degenerative cell layers. Variable sizes and shapes of Herbst corpuscles were detected alongside the papillary duct and near the glandular lobe of the PG. In transmission electron microscopic analysis, the cytoplasm of the intermediate cells contained a dense population of mitochondria, while the secretory and degenerative cells contained fewer mitochondria.

Histomorphology and ultrastructure of the proventriculus of the broad breasted white turkey (Meleagris gallopavo, Linnaeus 1758).

This study aims to examine the functional morphology of the proventriculus of the broad breasted white turkey (BBWT) (Meleagris gallopavo, Linnaeus 1758) using gross anatomy, light microscopy, gross/histomorphometric analysis, and scanning and transmission electron microscopy. The proventriculus was characterized internally by many elevated papillae with a mound, leafy flower, and lotus flower-shapes. Each papilla was enclosed by concentrically organized mucosal folds with distinct or indistinct proventricular gland openings on its top. Longitudinal folds and grooves at the junction of the proventriculus with the esophagus exhibited various sized and shaped openings of esophageal glands with irregular outlines. Histologically, the surface epithelium of the proventriculus was covered by a thin layer of cuticle, particularly evident at its junction with the gizzard. The lamina epithelialis and propria, and secretory units of proventricular lobules were infiltrated by aggregations of lymphocytes and lymphoid follicles (nodules). Variably shaped glandular lobules of proventricular glands occupied the submucosa, surrounded by thin strands of smooth muscle fibers derived from muscularis mucosa. Triangular, cuboidal, or columnar-shaped secretory oxyntico-peptic cells lined the secretory units. Many telocytes (pyramidal or fusiform-shaped cell bodies with lengthy telopodes) were observed in interstitial tissue. Further, two types of argyrophilic endocrine cells were identified within the glandular epithelium. The morphology of the M. gallopavo proventriculus reflects its dietary habits and behavior.

Lingual papillary system of the Egyptian mouse-tailed bat (Rhinopoma hardwickii): New insights to its feeding strategies using scanning electron microscope, and immunohistochemical analysis of keratin.

The current investigation described the ultrastructural and histological features of the lingual papillary system in 14 adult Rhinopoma hardwickii using scanning electron and light microscopy, and immunohistochemical staining of keratin. Three types of lingual papillae were recognized; one filiform mechanical and two gustatory (fungiform and circumvallate) of three directions: posterior, posteromedian, and median. Five filiform papillae subtypes were observed in four regions. The lingual tip had numerous giant filiform papillae. The lingual root was divided into two regions, an anterior papillary and a posterior non-papillary region that had only three circumvallate papillae in a triangular pattern. Histologically, the papillae exhibited detached thick keratin that spread over the dorsal epithelial surface. The dorsal surface of the root had stratified keratinized circumvallate papillae. Concerning the pan-cytokeratin (CK) antibody staining, moderate CK expression was observed in the superficial corneal layer of the apical lingual mucosa. While the superficial corneal cell layer in both body and root exhibited increasing of CK antibody. Morphometrically, there was a significantly increased, region-specific number of triangular, pointed filiform papillae, which were relatively increased in length and width. However, the round conical blunt filiform papillae and fungiform papillae were sparse. We concluded that our results showed distinguished adaptations in the lingual structure and its different papillae established on the nutritional and energetic demand of this bat species. This lingual adaptation is summarized in number, position, taste buds, direction, ultrastructural features, and histological that concerned with its ability in eating during the night flight. RESEARCH HIGHLIGHTS: Three types of lingual papillae; one mechanical (filiform) and two gustatory (fungiform and circumvallate) in which there were three circumvallate papillae on the posterior part of the lingual root in a triangular arrangement with an irregular surface, but the filiform papillae had five subtypes arranged in four regions. The lingual root was divided into two regions, an anterior papillary region and a posterior non-papillary region that only had three circumvallate papillae. Concerning staining with a pan-cytokeratin (CK) antibody, moderate CK expression was observed in the superficial corneal layer of the lingual mucosa of the tongue apex while the superficial corneal cell layer of the body and root exhibited increased expression of the CK antibody compared to the lingual apex.

A histological and immunohistochemical study on the parabronchial epithelium of the domestic fowl's (Gallus gallus domesticus) lung with special reference to its scanning and transmission electron microscopic characteristics.

The current study was designed to give complete histo-and immunohistochemical features of the parabronchial epithelium of domestic fowl's (Gallus gallus domesticus) lung with special reference to Scanning electron microscope (SEM) and mean transmission electron microscope (TEM) features. The lung exhibited variable-sized atrial openings encircled by exchange tissue zones. The parabronchial atrial chambers appeared as ovoid and polygonal-shaped that separated by the well-developed interatrial septum. The deep atrial lumens had blood vessels pierced by openings that represent the infundibula. The parabronchial blood capillaries meshwork was branched and exhibited ovoid-shaped air capillaries with numerous extravasated blood vessels. By TEM, there were several air capillaries and groups of squamous and endothelial respiratory cells and the squamous cells had oval nucleus with evenly distributed chromatin. The endothelial respiratory cells had few microvilli on their free surfaces. The parabronchial tubes opened into a group of widened atria that had smooth muscle bundles at the interatrial septa. The atrial chambers led to narrow infundibula. Moreover, the lining epithelium of parabronchi, atria, infundibula, and air capillaries was formed by simple squamous epithelium. Air capillary walls were lined by two types of respiratory cells (Types-I and II). Collagen fibers were concentrated within the tunica externa layers of the parabronchial blood vessels as well as, they were observed in CT interparabronchial septa. Immunohistochemically, the elastin immunoreactivity was detected around the parabronchial blood vessels, at the base of each parabronchial atria, and in the area encircling the alveolar-capillary walls. Our work concluded that there are a relation between the fowl's lifestyle and the surrounding environmental conditions.

Ultrastructural and histological descriptions of the oropharyngeal cavity of the rock pigeon Columba livia dakhlae with special refer to its adaptive dietary adaptations.

The current investigation gave a full ultrastructural and histological description of the oropharyngeal cavity of the rock-pigeon Columba livia dakhlae. Our study carried on 10 heads of adult rock pigeons by gross, scanning, and light microscopic examination, in addition to the immunohistochemical analysis of the cytokeratin of the dorsal surface mucosa epithelium and the proliferating cell nuclear antigen expression (PCNA) immunoreactivity. The pointed apex of the elongated tongue covered rostrally and laterally with numerous caudally directed scales-like papillae. The dorsal surface of the apex and body was covered with numerous caudally directed scales-like filiform papillae. The U-shaped transverse papillary crest carried caudally directed pointed triangular conical papillae with two giant papillae. The elevated caudal area of the lingual body contains little numbers of the opening of the lingual gland. The laryngeal mound is divided into two symmetrical halves by rostral fissure and caudal laryngeal fissure into the right and left half. There were four palatine ridges: two lateral, middle, and median ridges. The choanal field bounded by the two lateral palatine ridges and containing the median choanae. The rostral part of the palatine cleft bounded laterally by a line of the small caudally directed conical papillae, while the caudal part was free from these papillae. There was a little number of taste buds in the palate. The high magnification of the rostral part of the beak is covered with directed rods-like projections. In conclusion, the obtained results described the adaptations of the tongue and its belonged structures with the feeding demand of the rock-pigeon.

Comparative ultrastructural-functional characterizations of the skin in three reptile species; Chalcides ocellatus, Uromastyx aegyptia aegyptia, and Psammophis schokari aegyptia (FORSKAL, 1775): Adaptive strategies to their habitat.

The current investigation was planned utilizing SEM, histological, and furthermore cytokeratin immunohistochemical to give a full depiction of skin of three reptiles species; Chalcides ocellatus (Scincidae), Uromastyx aegyptia aegyptia (Agamidae), and Psammophis schokari aegyptia (Colubridae) captured from Egypt with various ecological environment. Our SEM results showed different scales covered epidermis of three reptile's species with diverse surface microstructure. Overlapped rhomboid scales with numerous lenticular sense organs with numerous pores and oberhäutchen layer with microridges in C. ocellatus. In U. aegyptia, scales were overlapped elliptical-shaped possess lens-like sense structure with several scattered pits and oberhäutchen layer with polygonal outlined cells. While in P. schokari aegyptia, smooth scales flattened with two large dome-shaped scale receptors/sensilla and lens-like sense structure, moreover polygonal-shaped micro-ornamentation in scale hinge joints were observed. Histologically, skin of three species had outer epidermis with stratum germinativum, stratum corneum (α-keratin, ß-keratin layer) capped by surface Oberhäutchen and inner dermis. Osteoderms were observed with dermis of C. ocellatus. There are marked variation within pigment cells types among examined species. Melanophores observed in dermal layer of C. ocellatus, while in U. aegyptia, three pigment cells in tegument dermis; melanophores, xanthophores, and iridophores whereas, P. schokari aegyptia had two forms of chromatophore cells (melanophores and iridophores) in dermis and few melanophores scattered between stratum germinativum cells. The highest cytokeratin immunostaining observed in epidermal cell layer of U. aegyptia aegyptia than two other species. Conclusion, dry scaly skin of reptiles reflects a great range of functional aspects and success to adapt with terrestrial life.

Ultrastructural comparison between the tongue of two reptilian species endemic in Egyptian fauna; Bosc's fringe-toed lizard Acanthodactylus boskianus and Sinai fan-fingered gecko Ptyodactylus guttatus.

The current observations focused on the ultrastructure comparison between the tongue of two reptile species endemic the Egyptian fauna; Bosc's fringe-toed lizard Acanthodactylus boskianus and Sinai fan-fingered gecko Ptyodactylus guttatus to exhibit the relationship between the lingual epithelium and its function according to their specific feeding strategy. A. boskianus possessed triangular elongated tongue with bifurcated tapering apex and wide base while; the P. guttatus had a triangular flattened tongue with conical shallow bifurcated apex and broad base. The ventral surface of the lingual apex of A. boskianus had transverse while in P. guttatus had two oval pads and median ventral groove. Both surfaces of the tongue of both examined species are covered by stratified squamous epithelium with great variability of degree of keratinization. The dorsal epithelium formed flattened and conical filiform papillae in A. boskianus, while in P. guttatus formed cylindrical papillae, conical, and tall filiform ones. Few taste buds are observed on the fore-tongue but increase on the mid-tongue of A. boskianus, while in P. guttatus, numerous taste buds are distributed on the fore-tongue and mid-tongue. Both surfaces of the laryngeal mound of both examined species provided with numerous of cilia and orifices of laryngeal gland. The present results confirmed that the tongue of A. boskianus acts as a chemoreceptor organ to follow pheromone trails of prey and mates. While in P. guttatus the tongue may play an important role in the feeding mechanism and act as a chemoreceptor organ.

Age-related ultrastructural features of the tongue of the rock pigeon Columba livia dakhlae in different three age stages (young, mature, and adult) captured from Egypt.

This study represents the first attempt to describe the ultra-structural and histological features of different three age-stages (young, mature, and adult) of rock pigeon Columba livia dakhlae captured from Egypt. The elongated tongue with round lingual apex carried a lingual nail rostrally on lingual tip. The current work gave a first record of numerous descriptions as in young and mature, small lingual nail formed from aggregation of lingual scales, but in adult, the nail formed from lingual membrane without any lingual scales. The current work gave full descriptive methods of shape, directions, and distribution of filiform-like papillae with lingual scales among three age-stages on dorsal surface and lateral border of apex and body. The taste buds present only on the tongue of mature and adult pigeon. The only one transverse row in young took the wide V-shape but, wide U-shape in mature and adult. The papillae on the papillary crest took triangular spoonful-like shape in young and mature, but in adult take the shape of blunt tubercles. The current work was the first record of the presence of the openings of lingual glands on concave dorsal lingual surface of giant papillae and papillae of papillary crest in adult stage. The lingual membrane was thin one membrane in young and three to four layers in mature but, in adult it is well-developed and formed from 10-11 layers. The supportive entoglossum cartilaginous was extended rostrally till the apical part.